【摘要】 目的:建立双波长高效液相色谱同时测定人血清中维生素A、E(VitA、VitE)浓度的方法。方法:移取血清,加入乙腈乙醇混合溶液及正己烷,旋涡混合、离心,分离正己烷层氮气吹干,残渣用流动相溶解后进样。流动相为甲醇-水(92∶8);流速1mL·min-1;柱温30℃,紫外检测器在325nm和292nm检测VitA和VitE。结果:VitA保留时间为2.63min,浓度线性范围50~800μg·L-1,r2=0.9995,平均回收率为97.3%(RSD<5%),最低检出量0.4ng(S/N>3);VitE保留时间为7.61min,浓度线性范围2~16mg·L-1,r2=0.9999,平均回收率为96.2%(RSD<6%),最低检出量2ng(S/N>3)。40例健康成人血清VitA平均值为(389.0±138.0)μg·L-1,VitE平均值为(10.2±2.5)mg·L-1。结论:双波长HPLC检测血清中VitA和VitE浓度的方法灵敏度高,回收率和重现性良好,操作快速、简便。更多还原

【Abstract】 OBJECTIVE To establish an HPLC assay for the determination of vitamin A(VitA) and vitamin E(VitE) in human serum and evaluate the nutritional value of the body’s of VitA and VitE.METHODS The serum was extracted with n-hexane after the protein in the serum was deposited with acetonitrile /alcohol.The layer of n-hexane was dried with nitrogen,and the residue was dissolved with mobile phase and determined by HPLC.VitA and VitE were separated on XTerra TM RP 18 5 μm, 3.9 mm×150 mm column,detected at 292 nm for VitE and 325 nm for VitA.Using methanol / water(92/8) as mobile phase when the column temperature was 30 ℃.RESULTS The linearity range of calibration curve of VitA was 50 -800 μg·L -1 (r2= 0.999 5).The average extracted recovery is 97.3%,RSD<5%.The linearity range of calibration curve of VitE was 2-16 mg·L -1 (r2= 0.999 9).The average extracted recovery was 96.2%,RSD<6%.The detection limit of VitA was 0.4 ng and VitE is 2 ng (S/N>3).The whole operation could be finished in less than 30 minutes.CONCLUSION The method is sensitive,rapid,accurate and simple.更多还原