【摘要】 目的:用高效液相色谱法测定盐酸伪麻黄碱(PSE)的血药浓度。方法:色谱柱为D iamonsil C18(4.6mm×200mm,5μm),流动相为0.5%硫酸胺-甲醇(80∶20),检测波长210nm,流速1.0mL.m in-1。结果:标准曲线的线性范围为3.125~20mg.L-1(r=0.999 3,n=7),低、中、高3种浓度的日内和日间RSD分别为4.31%,2.30%,2.07%(n=5)和5.83%,3.54%,4.16%(n=5),相对回收率分别为94.17%,101.22%,97.03%。结论:本方法简便、快速、准确、专一性好,可用于盐酸伪麻黄碱的血药浓度测定。更多还原

【Abstract】 OBJECTIVE To determine the plasma drug concentration of PSE in rats with RP-HPLLC method.METHODS Diamonsil C₁8（4.6 mm×250 mm,5 μm） was used and the mobile phase consisted of 0.5%ammonium sulfate-methanol（80∶20） at the flow rate of 1.0 mL·min^-1.The UV detection wave length was 210 nm.RESULTS The linear range was 3.125-200㎎·L^-1（r = 0.999 3,n= 7） and the limit of detection for PSE was 0.02 mg·L^-1.The precision of the intra-day and inter-day of three different concentration solutions were 4.31%,2.30%,2.07%（n= 5） and 5.83%,3.54%,4.16%（n= 5） respectively,and the extration recovery was 94.17%,101.22%,97.03% respectively.CONCLUSION The method is simple, rapid,sensitive,and reliable,and it is suitable for the determing of drug concentration of PSE in human plasma in clinical use.更多还原