【摘要】 目的在爪蟾卵母细胞中建立低密度脂蛋白受体基因表达的固醇调控报告系统,通过中药的干预培养,从基因调控水平上阐明中药调脂作用的机制,并为药物筛选提供一个可靠的实验平台。方法将绿色荧光蛋白报告基因构建在调控低密度脂蛋白受体基因表达的固醇调控元件的下游,经显微注射导入爪蟾卵母细胞,在分别用含姜黄素、茶多酚等中药的培养液中培养3d后,用荧光显微镜及荧光分析仪对绿色荧光蛋白的表达情况进行定性定量分析。结果姜黄素诱导了绿色荧光蛋白的表达。结论①姜黄素可以通过影响固醇调控元件的作用上调低密度脂蛋白受体基因的表达；②建立的固醇调控报告系统可作为降胆固醇药物筛选和作用机制研究的实验平台。更多还原

【Abstract】 OBJECTIVE To establish a sterol regulatory report system of LDLR gene expression in Xenopus oocytes,investigate the lipid- lowering mechanism of Chinese medicine and provide a stable experimental system for Hopolipidemic.METHODS A green fluorescence pro- tein(GFP)gene in the downstream of sterol regulatory element-1 was constructed,a plasmid named pLXRN-4SRE-fPA was abtained.This plasmid was introduced into the nucleus of Xenopus oocytes by microinjection;the oocytes were cultured in the medium containing different kinds of Chinese medicines such as curcumin,tea polyphenols,tanshinone,and ligustrazine,etc.After 3 d,the expression of GFP in the oocytes was detected by a fluorescence microscopc and GFP fluorescence analyzer.RESULTS The expression of GFP was obviously induced only in the curcumin group.CONCLUSION①Curcumin can obviously up-regulate the expression of LDLR gene by influencing on SRE-1;②The sterol regulatory,report system establishes in the Xenopus oocytes can be a system for study on the lipid-lowering mechanism of Chinese medicine and screening Hopolipidemic.更多还原