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RP-HPLC同时测定菝葜中山柰酚和槲皮素的含量

Determination of Kaempferol and Quercetin in Smilax china by RP-HPLC

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【作者】 舒孝顺高中洪杨祥良

【Author】 SHU Xiao-shun~(1),GAO Zhong-hong~(2),YANG Xiang-liang~(2)(1.Department of Biology & Foodstuff Industry,Changsha University of Science & Technology,Changsha 410076,China;2.Institute of Materia Medica,Huazhong University of Science and Technology,Wuhan 430074,China)

【机构】 长沙理工大学生物与食品工程系华中科技大学药物研究所华中科技大学药物研究所 长沙410074武汉430074

【摘要】 目的建立一种RP-HPLC测定菝葜山柰酚和槲皮素含量的方法,并测定其不同产地的含量。方法采用LichrospherC18柱,水-乙腈-磷酸-三乙胺(65∶35∶0.27∶0.45)作流动相,流速0.7 mL.min-1,二级管阵列检测器(DVD),检测波长365 nm。结果槲皮素,山柰酚与相邻杂质能很好分离,其分离度分别为1.96和5.32,理论塔板数以槲皮素峰和山柰酚峰计算,分别为5 121和6 673;在5~100 mg.L-1内峰面积与其浓度均呈良好的线性关系(r=0.999 4,0.999 6),样品回收率分别为97.58%和105.67%;两成分含量以湖南张家界的最高而湖北赤壁最低。结论该方法精密度、重现性和分离效果好,分析快速准确,适合于该药材及提取物中山柰酚和槲皮素含量的测定,不同产地两成分均存在一定的差别。

【Abstract】 OBJECTIVE To establish a RP-HPLC method for the determination of kaempferol and quercetin in Smilax china L.,and to investigate their contents in different places.METHODS The determination was performed on a Lichrospher C18 column(4.6 mm×250 mm,5 μm).The detection wavelength was at 365 nm,with the mobile phase of Water-Acetonitrile-Phosphoric-Triethylamine(65∶35∶0.27∶0.45) at the flow rate of 0.7 mL·min-1.RESULTS Kaempferol,quercetin and impurities were separated.The theoretical plate numbers of kaempferol and quercetin were 5 121 and 6 673,the resolution between kaempferol,quercetin and impurities were 1.96 and 5.32,respectively.All the parameters were suitable for determination.Both of kaempferol and quercetin showed a good linearity over the range of 5~100 mg·L-1(r=0.999 4,0.999 6),respectively,the average recoveries of kaempferol and quercetin were 105.67% and 87.58%,respectively.Their contents were highest in Zhang jia jie,Hunan and lowest in Chibi,Hubei.CONCLUSION The RP-HPLC method shows a good separation,reproducity and accuracy.It will be used for determining kaempferol and quercetin in herbs and extracts.The contents of kaempferol and quercetin are different in Smilax china L.from different places.

【关键词】 反相高效液相色谱法菝葜槲皮素山柰酚
【Key words】 RP-HPLCSmilax china L.quercetinkaempferol
  • 【文献出处】 中国药学杂志 ,Chinese Pharmaceutical Journal , 编辑部邮箱 ,2006年06期
  • 【分类号】R284.1
  • 【被引频次】11
  • 【下载频次】307
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