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La对UV-B辐射下大豆幼苗NRA和可溶性蛋白的影响

Effect of La3+ on soybean seedling nitrate reductase activity and soluble protein content under supplemental UV-B radiation

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【作者】 薛隽刘文张光生周青

【Author】 XUE Juan,LIU Wen,ZHANG Guang-sheng,ZHOU Qing(The Key Laboratory of Industrial Biotechnology,Ministry of Education,Southern Yangtze university,Wuxi 214036,China)

【机构】 江南大学工业生物技术教育部重点实验室江南大学工业生物技术教育部重点实验室 江苏无锡214036江苏无锡214036

【摘要】 在温室模拟增强紫外辐射UV-B(280~320nm)环境下,研究镧(La)对UV-B胁迫下大豆幼苗叶片硝酸还原酶活性(N itrate reductase activity,NRA)、可溶性蛋白、淀粉和可溶性糖含量的影响。结果显示,高低两个紫外强度剂量下,叶片NRA较对照组都有显著下降,处理5d后降幅分别达到40.9%和42.0%;而经过适宜浓度LaC l3(20mg/L)预处理后,降幅较UV-B单独处理组均有明显升高,分别为39.2%和38.9%。蛋白质、淀粉和可溶性糖含量变化与NRA相似,但是紫外辐射处理组蛋白含量变化较对照组呈现先升高后下降趋势。结果表明适宜浓度的La3+能够促进NRA以及UV-B胁迫下NRA活性的提高,缓解UV-B胁迫对植物的伤害。

【Abstract】 Effect of La3+ on the leaf nitrate reductase activity(NRA) and the contents of soluble protein,soluble sugar and starch in soybean seedlings were studied under elevated ultraviolet-B radiation(UV-B,280320nm).The result showed that NRA decreased significantly under the two different doses of UV-B treatment,with a decrease of 40.9% and 42.0% compared to the control after 5 days of treatment.But after pretreated with 20mg/L LaCl3,the NRA increased by 5%10% compared to that treated by UV-B radiation only.The same changes were found with contents of starch and soluble sugar as NRA.But protein content only had a little difference,which increased after UV-B treatment in the beginning 3 days and decreased in following 2 days.Our results indicated that 20mg/L LaCl3 had an effect to increase leaf NRA treated by UV-B,and alleviated seedling damage caused by UV-B.

【基金】 国家发改委稀土专项基金(IFZ051210);国家自然科学基金(30570323)
  • 【文献出处】 中国油料作物学报 ,Chinese Journal of Oil Crop Sciences , 编辑部邮箱 ,2006年03期
  • 【分类号】S565.1
  • 【被引频次】14
  • 【下载频次】180
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