【摘要】 目的:通过内毒素血症幼年大鼠小肠Bc l-2及Bax mRNA表达的变化,探讨小儿胃肠功能障碍的机制。方法:腹腔注射内毒素制备18 d龄大鼠内毒素血症模型,注射同等量生理盐水为对照组,分别取全部回肠,半定量RT-PCR法检测Bc l-2、Bax mRNA的表达。结果:正常对照和内毒素组小肠各时间点的Bc l-2 mRNA均为阴性表达,正常对照Bax mRNA表达较弱,内毒素组小肠各时间点的Bax mRNA表达均明显增强(P<0.01),死亡组与24 h之内其他各组的表达无显著差异,比72 h亚组表达明显增高(P<0.05)。内毒素组Bax与Bc l-2 mRNA表达的比值明显高于对照组。结论:内毒素通过调节基因Bc l-2、Bax mRNA表达的变化促进幼年大鼠小肠细胞凋亡可能为严重感染时胃肠功能障碍机制之一。更多还原

【Abstract】 Objective: To investigate the expressions of B-cell lymphoma-2(Bcl-2) and Bcl-2 associated X protein(Bax) mRNA in small intestines of infant rats with endotoxemia and to explore the mechanism of gastrointestinal dysfunction in children.Methods: The endotoxemic model of rat aged 18 days was established by intraperitoneal injection of endotoxin.The rats in control group were intraperitoneally injected with equal volume of saline.The entire ileum was sampled.The expressions of Bcl-2 and bax mRNA were detected by using semi-quantitive reverse transcriptase polymerase chain reaction.Results: The negative expression of Bcl-2 mRNA in small intestine was found at each time point both in control group and in endotoxemia group.The expression of bax mRNA in small intestine was weak in control group and significantly increased at each time point in endotoxemia group(P<0.01).No significant difference in bax mRNA expression was found between dead group and other subgroups within 24 hours.But bax mRNA expression in dead group was much higher than that in other subgroups at the 72th hour(P<0.05).The ratio of bax expression to Bcl-2 mRNA expression at each time point in endotoximia group was significantly higher than that in control group(P<0.01).Conclusion: Endotoxin may facilitate the apoptosis of small intestinal cells by regulating the expressions of Bcl-2 and bax mRNA,which may be one of the mechanisms of gastrointestinal dysfunction during severe infection.更多还原