【摘要】 目的:探讨血小板源性生长因子B(PDGF-B)基因转染修饰大鼠骨髓间充质干细胞(MSC)的可行性。方法:应用FAM标记重组真核表达载体系统(pcDNA3-PDGF-B),以脂质体法转染原代MSC,观察转染结果、表达情况及对靶细胞活力的影响。结果:MSC的基因转染成功,持续表达时间超过了8周,没有发现明显的细胞毒作用及对细胞活力的显著影响。结论:采用真核转染技术可以介导外源基因转染MSC,MSC是一种理想的基因载体细胞,可用于PDGF-B的基因治疗。更多还原

【Abstract】 Objective: To study the feasibility of transfecting platelet-derived growth factor-B（PDGF-B） gene to bone marrow mesenchymal stem cells（MSCs）.Methods: Recombinant eukaryotic expression carrier system(pcDNA₃-PDGF-B) was marked with carboxyfluorescein（FAM）.Original MSCs were transfected by using liposome technique.The results of transfection and the activity of the target cell were determined.Results: Bone marrow MSCs were successfully transfected with pcDNA₃-PDGF-B.The expression of exogenous gene continued over 8 weeks.No cell toxicity and obvious influence on cell activity was found.Conclusion: MSCs can be efficiently transfected with exogenous gene by using eukaryotic transfection technique.MSCs are ideal cells for cell-mediated transfection and can be used in PDGF-B gene therapy.更多还原