【摘要】 目的:观察哮喘大鼠骨形态构建蛋白2,4(BMP-2,BMP-4)表达情况。方法:SD大鼠45只,随机分为2组,对照组(Ⅰ组)为健康大鼠;哮喘模型组(Ⅱ组)为卵清白蛋白(OVA)致敏后吸入激发建立的模型。HE染色后图像分析检测大鼠气道形态学参数,免疫组化方法用于BMP-2,BMP-4表达定位,W estern蛋白印迹法测定BMP-2,BMP-4含量。结果:Ⅱ组与Ⅰ组相比,支气管内径缩小,平滑肌层增厚(P<0.05);BMP-2表达增加12.3%,BMP-4表达减少7.9%(P<0.05)。结论:哮喘大鼠肺内BMP-2,BMP-4可能参与哮喘病理生理变化调控。更多还原

【Abstract】 Objective: To investigate the expressions of bone morphogenetic protein-2(BMP-2) and BMP-4 in the lungs of rats with asthma.Methods: Forty-five SD rats were randomly divided into 2 groups: group 1(control group) and group 2(asthma group).The asthmatic rat model was established by ovalbumin(OVA) sensitization and challenge.The changes of airway,the expressions of BMP-2 and BMP-4,and the contents of BMP-2 and BMP-4 were determined by using image analysis system,immunohistochemical method,and Western Blotting,respectively.Results: Compared with group 1, the bronchial internal diameter decreased and the thickness of bronchial smooth muscle increased in group 2(P<0.05).The expression of BMP-2 was significantly higher than that in group 1 [CM(5:46](42.1±6.2 vs.29.8±8.4%,P<0.05),and the expression of BMP-4 in group 2 was significantly lower than that in group 1 (11.5±4.2 vs.19.4±5.3%,P<0.05).Conclusion: The increase in BMP-2 expression and the decrease in BMP-4 expression may participate in the pathogenesis of asthma in rats.更多还原