【摘要】 目的:研究不同缺血预处理方法对在体兔颌下腺缺血再灌注(I/R)损伤的保护作用。方法:血管夹夹闭兔颌下腺动、静脉1 h后恢复血运建立颌下腺I/R损伤的动物实验模型。施加缺血预处理(IP)因素,夹闭颌下腺动、静脉5 m in和10 m in,相对应放开血管夹恢复血运10 m in和15 m in,制作两种IP模型IP1和IP2,然后再进行I/R损伤实验。检测I/R组、IP1组、IP2组颌下腺组织缺血再灌注1,2,3,5 h超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。组织标本HE染色。结果:I/R后SOD活力升高,MDA含量上升。IP2组SOD活力显著高于I/R组,但低于IP1组。IP2组MDA含量显著低于I/R组,但高于IP1组。HE染色结果IP1组颌下腺损伤程度低于IP2组,IP2组伤程度低于I/R组。结论:IP可以减轻I/R对颌下腺的损伤,IP1较IP2减轻I/R对颌下腺的损伤的作用明显。更多还原

【Abstract】 Objective: To evaluate the effects of different ischemic preconditionings(IP) on ischemia/reperfusion(I/R) injury of submandibular glands.Methods: I/R injury rabbit model was established by occluding the arteries and veins of submandibular gland with vascular clips for 1 hour and then performing reperfusion.Two types of IP rabbit models,IP1 and IP2,were established by occluding the arteries and veins for 5 and 10 minutes and then performing reperfusion for 10 and 15 minutes before I/R injury,respectively.The activity of superoxide dismutase(SOD) and the amount of malondialdehyde (MDA) in the submandibular gland were determined 1 hour,2 hours,3 hours,and 5 hours after reperfusion.The tissues were stained with HE method.Results: The activity of SOD and [CM(5:46]the amount of MDA in the submandibular glands increased after I/R injury.The activity of SOD in IP2 group was higher than that inI/R group but lower than that in IP1 group.The amount of MDA in IP2 group was significantly lower than that in I/R group but higher than that in IP1 group.The results of HE staining showed that the injury of submandibular gland in IP2 group was more severe than that in IP1 group but less severe than that in I/R group.Conclusion: IP has a protective effect on submandibular gland injury induced by I/R.The protective effect of IP1 is better than that of IP2.更多还原