【摘要】 目的:研究人巨细胞病毒(HCMV)UL137基因在先天巨细胞病毒感染临床低传代分离株中的多态性。方法:选择经荧光定量PCR方法检测HCMV-DNA为阳性的临床分离株进行涵盖UL137全序列的UL136,UL138基因全序列PCR扩增,对扩增阳性的标本进行全序列的测序,核实测序结果,拼接出UL137基因编码序列,进行相关分析。结果:经分析获得22株HCMV临床分离株UL137 ORF核苷酸序列,核苷酸变异分布于整个编码区,但主要集中在5′端220~290 bp处,均为核苷酸碱基替换,无插入及移码突变。在UL137预测编码蛋白氨基酸序列中,新增2个半胱氨酸位点,导致17株临床分离株在71~76位氨基酸出现了N相连豆蔻酰化位点(MYR)的新增,及第90~92位氨基酸硫化cAMP位点的相应缺失。结论:HCMVUL137基因在临床低传代分离株中高度保守,但存在一定的多态性。更多还原

【Abstract】 Objective: To investigate the polymorphism of human cytomegalovirus(HCMV) UL137 gene in low passage clinical isolates.Methods: The entire HCMV UL136 and UL138 gene regions,which covered the entire UL137 gene region,of clinical isolates in which positive HCMV-DNA was detected by using fluorescence quantitative polymerase chain reaction(FQ-PCR) were amplified by polymerase chain reaction(PCR).Amplified PCR products were sequenced,and the encoding sequence of UL137 gene was spliced. Results: The nucleotide sequence of UL137 open reading frame was obtained from 22 clinical isolates.The nucleotide mutations mainly located between 220 to 290 bp and were all base substitutions,but neither insertions nor deletions were found.In UL137 amino acid sequences,2 new cysteines were added,which led to the addition of myristoylation site and corresponding deletion of sulfation cAMP site in 17 clinical isolates.Conclusion: HCMV UL137 gene in low-passage clinical isolates was highly conserved, regardless of their polymorphism.更多还原