【摘要】 目的对海洋真菌代谢产物中抗癌活性成分进行分离和结构鉴定,并对得到的化合物进行体外抗癌活性评价。方法用硅胶、SephedaxHL-20、ODS柱色谱及制备高效液相色谱等方法进行分离,通过化合物的理化性质及各种光谱技术鉴定化合物的结构。采用MTT法测定化合物体外抗癌活性。结果从真菌菌丝体的丙酮提取物中分离得到两个生物碱类化合物和两个甾醇类化合物,分别鉴定为viridicatol(1)、sclerotiamide(2)、22E-5α,6α-环氧麦角甾-8(14),22-二烯-3β,7α-二醇(3)、22E-5α,8α-桥二氧麦角甾-6,22-二烯-3β-醇(4)。化合物3和4在50μg·mL-1的质量浓度下可以明显地抑制HepG2、人乳腺癌MCF-7细胞和NCI-H460肿瘤细胞的生长。结论化合物3和4有明显的体外抗癌作用。更多还原

【Abstract】 Aim To isolate the anti-cancer constituents form a marine fungus 96F197,determine the structure and evaluate their anti-cancer bi oa ctivities in vitro.Methods Compounds were isolated by sili ca gel,ODS column chromatography,size exclusion column chromatography （Sephadex LH-20）,and reversed-phase HPLC purification.Structural elucidation was achiev ed by physic-chemical constants and spectroscopic analysis.MTT was used to eval uate the bioactivities in vitro.Results Two alkaloids and two sterols were isolated from acetone extracts of mycelium of strain 96F197 and elucidated as viridicatol（1）,sclerotiamide（2）, 22E-5α,6α-epoxyergosta-8（14）,22-diene-3β,7α-diol,22E-5α,8α-epidioxyergosta-6,22-dien-3β-ol（4 ）.Coumpoud 3 and 4 can evidently inhibit the gro wth of cancer cells HepG2,MCF-7 and NCI-H460 at the concertration of 50 μg·m L ^-1.Conclusion Compound 3 and 4 show anticancer bioactivities in vitro.更多还原