【摘要】 目的:采用反相高效液相色谱-蒸发光散射检测法(HPLC-ELSD)同时测定枸橼酸钾中枸橼酸和钾的含量。方法:采用Synergi Fusion-RP80色谱柱(250 mm×4.6 mm,4μm),以甲醇-水(含甲酸1%)(5∶95)为流动相,流速为1 mL/min,柱温为室温;ELSD检测条件:漂移管温度为40℃,雾化气体压力为3.5 bar。结果:枸橼酸在0.24~11.85μg范围内,进样量与峰面积呈良好的线性关系,r=0.999 2,平均回收率为101.9%(n=9),RSD为2.5%(n=6);钾在0.07~3.61μg范围内,进样量与峰面积呈良好的线性关系,r=0.999 4,平均回收率为101.8%(n=9),RSD为1.3%(n=6)。结论:本方法可用于枸橼酸钾中枸橼酸和钾的同时定量测定。更多还原

【Abstract】 Aim:To simultaneously determine citric acid and potassium in potassium citrate by HPLC-ELSD.Methods: The developed method was based on the separation of citric acid and potassium on Synergi Fusion-RP80 column(250 mm×4.6 mm,4 μm) at ambient temperature using methanol-water(containing 1% formic acid)(5∶95) as the mobile phase and a flow rate of 1 mL/min;ELSD was used as the detector with nebulizing air pressure of 3.5 bar and the drift tube temperature of 40 ℃.Results: A linear range of the injected amount of citric acid between 0.24 μg and 11.85 μg over the responses and the mean recovery of 101.9%(n=9)with RSD of 2.5%(n=6)were obtained.And it was also found that a linear range of potassium between 0.07 μg and 3.61 μg with the mean recovery of 101.8%(n=9)with RSD of 1.28%(n=6)for potassium existed.Conclusion: A simple and accurate method was established for the simultaneous determination of potassium and citric acid in potassium citrate and its sustained-release tablets.更多还原