【摘要】 目的研究镉对卵巢子宫内膜雌激素受体(ER)及HSP 90α表达的影响,探讨其内分泌干扰作用机制。方法40只成年雌性Wistar大鼠按体重分层随机分为4组,每组10只,镉(Cd2+)染毒剂量分别为0、1.25、2.5、5.0 mg Cd2+/(kg.d),皮下注射,每周5 d连续4周。于动情间期将大鼠处死;用免疫组化染色法观察卵巢间质和子宫内膜ER表达以及子宫内膜HSP 90α表达。结果高剂量组卵巢间质ER表达显著降低于其他各剂量组(P<0.05);各染毒组子宫内膜ER表达与对照组差异无统计学意义(P>0.05),其中高、中剂量组显著低于低剂量组(P<0.05);各染毒组子宫内膜HSP 90α表达与对照组差异无统计学意义(P>0.05)。结论本实验条件下,高剂量组染镉可使卵巢间质ER表达显著下降,而各染毒组子宫内膜ER表达与对照组比较差异无统计学意义,其在镉的内分泌干扰作用机制中镉对ER表达影响的意义值得进一步研究;镉不会通过增加子宫内膜HSP 90α的表达来干扰内分泌系统。更多还原

【Abstract】 Objective This work was designed to analyze the cadmium effects on expression of estrogen receptor and HSP90 α in both the ovary and the uterus in rats,and to determined mechanisms of sex hormone-disruption of cadmium. Methods 40 female rats were weighed,weight-ranked,and assigned randomly to four groups and were dosed subcutaneously with CdCl₂ at doses of 0,1.25,2.5,and 5.0 mg Cd²⁺/（kg·d）,5 days/wk,for 4 weeks.The rats were killed on the day of the diestrus stage during the 4 day period after administration.Expression of ER and HSP90 α in both ovary and uterus were observed.Results Intensity of ER immunostaining in stroma of ovaries of the 5.0 mg Cd²⁺/kg group were significantly decreased compared with other groups（（P<0.05）.）Intensity of ER immunostaining in endometria of the CdCl₂ administration groups were not significantly different from the control group（P>0.05）.Intensity of ER immunostaining in endometria of the 5.0 mg Cd²⁺/kg group and the 2.5 mg Cd²⁺/kg group were significantly different from the 1.25 mg Cd²⁺/kg group（P<0.05）.Intensity of HSP90 α immunostaining in endometria of the CdCl₂ administration groups were not significantly different from the control group（P>0.05）.Conclusion The role of ER in the mechanism of endocrine disruption of cadmium might be worth of furter in study in the future.Cadmium can not increase the intensity of HSP90 α expression in endometria,so increasing the intensity of HSP90 α expression in endometria is not one of the mechanisms of endocrine disruption of cadmium in rats.更多还原