【摘要】 目的研究龟板的体外抗氧化活性,为抗衰老药物的筛选提供参考。方法将龟板分别用石油醚、乙酸乙酯、95%乙醇等极性依次递增的溶剂进行提取得不同提取部位溶出物,用DPPH法进行体外抗氧化活性检测,并与抗坏血酸进行比较。结果龟板95%乙醇提取部位溶出物与石油醚、乙酸乙酯部位提取物相比抗氧化活性均存在显著性差异(P<0.01),且以95%乙醇提取部位溶出物体外抗氧化活性最强,其EC50与抗坏血酸比较相差2.38个数量级。结论龟板95%乙醇提取部位溶出物具有很强的体外抗氧化活性,可作进一步研究。更多还原

【Abstract】 OBJECTIVE:To study the antioxidant activities of Plastrum Testudinis in vitro so as to provide references for the optimization of antiaging agents.METHODS:Extracts from different parts were extracted from Plastrum Testudinis with polarity progressive solvents in order:petroleum ether,ethyl acetate and 95% ethanol,respectively.Then in vitro antioxidant activities of the extracts were detected by DPPH method and compared with antiscorbic acid(Vit C).RESULTS:Significant differences were noted between the extracts by 95%ethanol ether and those by petroleum ether or ethyl acetate in antioxidant activities(P<0.01),with that extracted by 95% ethanol showing the highest antioxidant activities.2.38orders of magnitude difference was noted compared to Vit C in terms of EC 50 .CONCLUSION:The extracts of Plastrum Testudinis extracted by95%ethanol exhibits a strong antioxidant activity in vitro,which remains to be further studied.更多还原