【摘要】 目的比较2种方法测定3种抗癫痫药血清浓度的相关性。方法76例癫痫患者服药后采用高效液相色谱法和荧光偏振免疫法分别测定其血清浓度,并比较测定结果。结果以高效液相色谱法测定结果(X)与荧光偏振免疫法测定结果(Y)所作线性回归方程如下Y卡马西平=0.1851+0.9550X(r=0.9459);Y苯妥英钠=—1.418+1.136X(r=0.9562);Y苯巴比妥=—0.1273+0.9656X(r=0.9512),2种方法在统计学上无显著性差异(P>0.05)。结论高效液相色谱法与荧光偏振免疫法测定3种抗癫痫药血清浓度具有相关性,2种方法均可用于3种抗癫痫药血清浓度的常规监测。更多还原

【Abstract】 OBJECTIVE : To compare the relativity of determination of 3 antiepileptics: carbamazepin , phenytoin and phenobarbital, in human serum between HPLC and FPIA.METHODS: 76 epileptic patients were administered with carbamazepin ,phenytoin and phenobarbital respectively and the serum concentrations were determined by HPLC and FPIA respectively, and the results were compared. RESULTS : The regression equation of the results determined by HPLC (X)and FPIA(Y)were :for carbamazepin,Y=0.1 851+0.9 550X(r=0.9 459);for phenytoin,Y=—1.418+1.136X(r=0.9 562);for phenobarbital,Y=—0.1 273+0.9 656X(r=0.9 512).There were no significant statistical differences between the results determined by two methods(P>0.05).CONCLUSIONS : There is good relativity between human serum concentrations of carbamazepin ,phenytoin and phenobarbital determined by HPLC and FPIA. Both methods can be used for routine determination of these three kinds of antiepileptics.更多还原