【摘要】 目的观察静脉联合应用美托洛尔、胺碘酮对缺血/再灌注大鼠快速室性心律失常和心肌梗死范围的影响。方法Sprague-Dawley(SD)大鼠40只麻醉后,依缺血前所用药物不同,随机分为对照组、美托洛尔组、胺碘酮组、胺碘酮+美托洛尔组,复制缺血/再灌注模型,给药20min后,行缺血7min后,再灌注10min,观察再灌注期间室性心动过速和心室颤动的发生;然后再缺血30min,再灌注120min,观察心肌梗死/缺血区域面积比值,并经颈内动脉监测血压和心率。结果在观察过程中,各用药组血压、心率改变没有显著性差异。大鼠缺血/再灌注前静脉注射美托洛尔、胺碘酮显著减少缺血再灌注的室性心动过速,而胺碘酮+美托洛尔组进一步减少(P<0.01);同样,胺碘酮+美托洛尔组显著减少了再灌注诱发的总的心室颤动的发生(P<0.05),美托洛尔组、胺碘酮组没有统计学差异;不可逆心室颤动的发生,与胺碘酮组比较,美托洛尔组显著下降,胺碘酮+美托洛尔组较美托洛尔组进一步降低,但没有显著差异。美托洛尔组、胺碘酮组均减少了心肌梗死/缺血区域面积比率(P<0.05),胺碘酮+美托洛尔组则进一步降低(P<0.01)。结论联合应用美托洛尔和胺碘酮静脉注射可以进一步减少缺血/再灌注快速室性心律失常发生率,减少心肌梗死范围。更多还原

【Abstract】 Objective To investigate whether administration of metoprolol and amiodarone in ischemic/reperfusion rats can reduce the ventricular arrhythmia and myocardial infarction(MI) area. Methods 7-min ischemic and 10-min reperfusion after injection of metoprolol, amiodarone or combined from femoral vein in 40 anesthetized rats were subjected and the incidence of ventricular tachycardia (VT) and ventricular fibrillation (VF) were observed. Then 30-min ischemic and 120-min reperfusion and the myocardial infarction/ischemic area was assessmented in rats. During the observed procedure, the hemodynamics was monitored too. Results Among metoprolol-, amiodarone- or combined groups, there were no significant changes in hemodynamics.The intervene with metoprolol and amiodarone, reduced the incidence of ischemic-induced VT from 100% (control) to 65% and 62% respectively, and the combined group decreased to 42%(P<0.05). Also the incidence of reperfusion-induced lethal VF significantly decreased in metoprolol and comined group(23%,17% vs 33%,all P<0.05) . Amiodarone and metoprolol groups decreased the MI/ ischemic extent ratio (P<0.05), and the combined group decreased more significantly(P<0.01). Conclusions This may be a new beneficial role of combined metoprolol and amiodarone in decreasing cardiovascular mortality during ischemic/reperfusion.更多还原