【摘要】 目的研究中药柴芩温胆汤对慢性轻度不可预见性应激和孤养结合抑郁模型小鼠行为的影响。方法将60只雄性小鼠随机分为空白对照组、模型组、阿米替林对照组、柴芩温胆汤低剂量组、柴芩温胆汤高剂量组,每组12只。用抖笼法检测小鼠的行为学改变。结果抑郁模型小鼠的自主活动(82.50±14.98)次与空白对照组(336.00±46.85)次比较明显减少(P<0.01),其激惹次数(5.75±0.95)次与空白对照组比较显著增加(P<0.01);高、低剂量柴芩温胆汤、阿米替林均能明显改善小鼠的自主活动和降低激惹次数,但在改善自主活动方面柴芩温胆汤高剂量(323.75±63.40)次作用更为显著,与阿米替林组(254.25±28.34)次和柴芩温胆汤低剂量组(251.50±15.87)次均差异有显著性(P<0.01);而在降低激惹次数方面阿米替林(0.28±0.60)次和高剂量柴芩温胆汤(0.25±0.58)次作用相当(P>0.05),与低剂量柴芩温胆汤(1.25±0.50)次比较均差异有显著性(P<0.01)。结论中药柴芩温胆汤能明显改善抑郁模型小鼠的行为,具有抗抑郁作用,并存在一定的量效关系。更多还原

【Abstract】 ObjectiveTo study the effect of Chaiqin Wendan Decoction(CWD)on behaviors of mice under the conditions of chronic unpredictable stress and separation. Methods60 male mice were randomly allocated to five groups as follows:normal control group(NC) ,model group,amitriptyline control group,CWD groups which was divided into low dose and high dose.Every group had 12 mice.The changes of behaviors were detected by jiggle-cage method for all mice. ResultsCompared with other groups,depression model group was significantly lower on autonomic activity number(P<0.01),but obviously higher on irritation number(P<0.01). Compared with amitriptyline control group and low dose group of CWD ,autonomic activity number in the high dose group of CWD obviously increased(P<0.01). Compared with low dose group of CWD,irritation number in the high dose group of CWD and amitriptyline control group decreased(P<0.01),but there was no difference between high dose group of CWD and amitriptyline control group.ConclusionCWD can reverse the behaviors of chronic stress-induced depression mice,and the high dose group of CWD more significant.更多还原