【摘要】 目的观测白松片对抑郁模型大鼠海马睫状神经生长因子(CNTF)及其mRNA表达水平的影响,探讨白松片的抗抑郁作用的机制。方法将大鼠随机分为正常组、模型组、白松片组、氟西汀组,采用连续21d慢性轻度不可预见性应激配合孤养复制抑郁模型。运用免疫组化和原位杂交方法探讨白松片对抑郁模型大鼠海马神经元细胞CNTF、CNTFmRNA表达的影响。结果白松片组大鼠海马神经元CNTF免疫反应阳性细胞数目增多,神经元平均灰度值降低,其中CA1区(105.14±7.21),CA3区(104.47±6.05),DG区(108.18±7.56);CNTFmRNA杂交阳性信号增加,神经元平均灰度值降低,其中CA1区(182.14±12.68),CA3区(176.82±11.12),DG区(175.98±12.15)。与模型组比较,差异有显著性(P<0.05或P<0.01)。结论白松片增加抑郁模型大鼠海马CNTF、CNTFmRNA的表达,可能是其抗抑郁作用的分子机制之一。更多还原

【Abstract】 ObjectiveTo observe the effect of Baisong tablets on the level of Ciliary neurotrophic factor(CNTF) and CNTF mRNA in hippocampus of chronic stress depression rats. MethodsForty male Sprague-Dawley rats were randomly allocated to four groups: control group, model group, fluoxetine group and Baisong group. All rats except the control group were singly housed and exposed an unpredicted sequence of mild stressor.The level of CNTF and CNTF mRNA in CA1 and CA3 pyramidal and dentate gyrus of hippocampus were investigated with immunohistochemistry technique and in situ hybridization. ResultsCompared with model group, the CNTF like immunoreactivity CA1:105.14±7.21,CA3:104.47±6.05, DG:108.18±7.56 and signals of CNTF mRNA in situ hybridization CA1:182.14±12.68, CA3:176.82±11.12, DG:175.98±12.15 in the hippocampal neurons of Baisong group were significantly increased(P<0.05,P<0.01).ConclusionThese results show that Baisong tablets can significantly increase the level of CNTF and CNTF mRNA in the hippocampal neurons of depression model rats and possess antidepressant effect.更多还原