【摘要】 为研究中草药黄芪、板蓝根作为抗病毒药物的效果及初步机理,利用细胞病变(CPE)抑制实验测定黄芪、板蓝根单独使用及1：1联合使用在PK-15单层细胞上对猪细小病毒(PPV)的抑制作用。结果表明：板蓝根单独使用时体外对PPV有明显的抑制作用,其对PPV的最小直接杀灭浓度为0．62mg/mL,最小阻断浓度为0．31mg/mL；而黄芪单独使用时对PPV无明显的抑制作用。板蓝根、黄芪1：1联合使用时对PPV的抑制作用显著增强,其对PPV的最小直接杀灭浓度提高为0．31mg/mL,最小阻断浓度提高为0．075mg/mL。更多还原

【Abstract】 To study the anti-PPV(porcine Parvovirus)activity and mechanism of Radix Isatidis and Radix Astragali,Radix Isatidis and Radix Astragali were used separately and then mixed to challenge the PPV(porcine Parvovirus)cultured in PK-15 cell based on the inhibition to cytopathogenic effect(CPE).Minimal inhibitory concentration(MIC)and minimal stop concentration(MSC)had been determined directly and indirectly.The results showed that the anti-PPV cutivities of 1:1 mixture of Radix Astragadi and Radix Isatidis were significantly higher than that of Radix lsatidis,their MIC is 0.31 mg/mL, and their MSC is 0.075 mg/mL.更多还原