【摘要】 应用巢式聚合酶链反应(NestedPCR)-限制片段长度多态性(restrictionfragmentlengthpolymor-phism,RFLP)方法对微小隐孢子虫(C.parvum)、安氏隐孢子虫(C.andersoni)和火鸡隐孢子虫(C.melea-grides)的鉴别进行了研究。结果显示C.parvumBOCC2、C.andesoniBOCC2和C.meleagridesCHCC1扩增产物片段大小分别为830bp、828bp和828bp,扩增产物分别经VspⅠ酶切后形成3种不同的RFLP图谱,根据RFLP图谱可鉴别C.parvum、C.andersoni和C.meleagrides。本研究为我国隐孢子虫的分类和隐孢子虫病的分子流行病学研究打下了良好基础。更多还原

【Abstract】 Differentiation of Cryptosporidium parvum,C.andersoni and C.meleagrides was studied by Nested PCR-RFLP analysis of the SSU rRNA gene.The fragments of 830 bp,828 bp and 828 bp were amplified by Nested PCR from the C.parvum BOCC2,C.andesoni BOCC2 and C.meleagrides CHCC1 respectively.Three different kinds of RFLP restriction maps were formed after PCR products were digested by VspⅠrespectively.C.parvum,C.andersoni and C.meleagrides can be differentiated according to RFLP restriction maps.The study undertaked in the present experiment was suitable for the taxonomy of Cryptosporidium and molecular epidemiology survey of Cryptosporidiosis.更多还原