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不同放栓处理对FSH超排奶山羊发生未成熟黄体退化的影响

Effect of Different CIDR-G Insertion Protocols on Premature Luteal Regression in FSH-Superovulated Guanzhong Dairy Goats

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【作者】 高志敏杨炜峰杨春荣窦忠英

【Author】 GAO Zhi-min,YANG Wei-feng,YANG Chun-rong,DOU Zhong-ying*(Shaanxi Stem Cell Engineering and Technology Center,Northwest Agriculture and Forestry University,Shaanxi Yangling 712100,China)

【机构】 西北农林科技大学陕西省干细胞工程技术研究中心西北农林科技大学陕西省干细胞工程技术研究中心 陕西杨凌712100陕西杨凌712100

【摘要】 对24只关中奶山羊采用“促卵泡素+前列腺素+孕酮”激素组合进行超排。依据不同的放栓处理,将其分为2组,每组12只。组Ⅰ在放栓后第15天早去栓,组Ⅱ在首次放栓后的第8天早换新栓,并于第15天晚去栓。结果表明:两组间平均黄体数和平均采胚数差异均不显著(P>0.05);两组间平均大卵泡数差异显著(P<0.05)。组Ⅰ有3只山羊发生未成熟黄体退化现象,2只伴有大卵泡;组Ⅱ有2只山羊发生未成熟黄体退化现象,且伴有大卵泡。对正常黄体和未成熟退化黄体的结构分析发现,未成熟退化黄体的特点是各种类型细胞萎缩,细胞间存在大量胶原纤维;大黄体细胞占优势,而小黄体细胞、成纤维细胞和上皮细胞很少见。

【Abstract】 24 goats were superovulated using FSH,progesterone(P4)and PGF2α.All animals were equally divided into two groups depending on different CIDR-G insertion protocols.GroupⅠwas removed CIDR-G in the morning on day 15,groupⅡwas changed to a new CIDR-G in the morning on day 8 and was removed it in the evening on day 15.the number of corpus luteum(CL)and transferable embryos(P > 0.05)was not significantly different between two groups.The number of big follicular(P < 0.05)was significantly different.The incidence of premature luteal regression is 25%(3/12)of the goats in GroupⅠand 16.7%(2/12)in GroupⅡ.There were 2 premature luteolysis goats with big follicular in GroupⅠand 0 goats in GroupⅡ.From comparison of the histology and ultrastructure of normal CL and premature luteolysis CL,the morphological characteristics of premature luteolysis CL was that all kinds of cells were atrophy;there were lots of collagen fiber among cells;the high percentage cells were large luteal cells,and were rare to be observed small luteal cells,fibroblast cells and epithelial cells.

【基金】 科技部“863”项目(2002AA216161);教育部重大科技专项(03160)
  • 【文献出处】 中国畜牧杂志 ,Chinese Journal of Animal Science , 编辑部邮箱 ,2006年19期
  • 【分类号】S827
  • 【下载频次】82
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