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基因型和有机附加物对小白菜花药培养胚状体诱导的影响
Influences of genotypes and additive organic matters on embryogenesis of anther culture in pak-choi(Brassica campestris ssp. chinensis)
【摘要】 对13个小白菜品种进行花药培养,结果表明:在B5+谷氨酰胺0.8g/L+蔗糖10%固体培养基上有11个诱导出小孢子胚。各品种间小孢子胚产量差别较大,每100枚花药产胚数(诱导率)在0.11%-17.62%之间。比较B5培养基中蔗糖、有机物浓度和谷氨酰胺配比结果表明,B5+2倍B5有机物+谷氨酰胺0.4g/L+蔗糖10%+琼脂0.8%效果较好,小孢子胚最高诱导率达103.91%。
【Abstract】 Anthers from 13 varieties of pak-choi were cultured in B5 medium with 10% sucrose and 0.8 g/L L-glutamine, microspore-derived embryoids and regenerants were obtained from 11 varieties. The yield of microspore-derived embryoids (No. of embryoids per 100 anthers)varied from 0.11% to 17.62% among varieties. The effect of sucrose concentrations and additive organic matters in B5 medium on embryogenesis was investigated. The results showed that B5 medium with 10% sucrose and 0.4 g/L L-glutamine was optimal,with the highest efficiency of induction of 103.91%.
【Key words】 Pak-choi; Anther culture; Efficiency of induction; Plant regeneration;
- 【文献出处】 中国瓜菜 ,China Cucurbits and Vegetables , 编辑部邮箱 ,2006年02期
- 【分类号】S634.3
- 【被引频次】22
- 【下载频次】225