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基因型和有机附加物对小白菜花药培养胚状体诱导的影响

Influences of genotypes and additive organic matters on embryogenesis of anther culture in pak-choi(Brassica campestris ssp. chinensis)

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【作者】 蒋武生耿建峰张晓伟原玉香韩永平姚秋菊

【Author】 JIANG Wu-sheng, GENG Jian-feng, ZHANG Xiao-wei, YUAN Yu-xiang, HAN Yong-ping, YAO Qiu-ju (Institute of Bio-technology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China)

【机构】 河南省农业科学院生物技术研究所河南省农业科学院生物技术研究所 郑州450002郑州450002

【摘要】 对13个小白菜品种进行花药培养,结果表明:在B5+谷氨酰胺0.8g/L+蔗糖10%固体培养基上有11个诱导出小孢子胚。各品种间小孢子胚产量差别较大,每100枚花药产胚数(诱导率)在0.11%-17.62%之间。比较B5培养基中蔗糖、有机物浓度和谷氨酰胺配比结果表明,B5+2倍B5有机物+谷氨酰胺0.4g/L+蔗糖10%+琼脂0.8%效果较好,小孢子胚最高诱导率达103.91%。

【Abstract】 Anthers from 13 varieties of pak-choi were cultured in B5 medium with 10% sucrose and 0.8 g/L L-glutamine, microspore-derived embryoids and regenerants were obtained from 11 varieties. The yield of microspore-derived embryoids (No. of embryoids per 100 anthers)varied from 0.11% to 17.62% among varieties. The effect of sucrose concentrations and additive organic matters in B5 medium on embryogenesis was investigated. The results showed that B5 medium with 10% sucrose and 0.4 g/L L-glutamine was optimal,with the highest efficiency of induction of 103.91%.

【基金】 国家“863”项目(2001AA241125);河南省重点攻关项目(0123012200)
  • 【文献出处】 中国瓜菜 ,China Cucurbits and Vegetables , 编辑部邮箱 ,2006年02期
  • 【分类号】S634.3
  • 【被引频次】22
  • 【下载频次】225
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