【摘要】 目的建立一种快速、准确、特异、定时检测鲍曼不动杆菌TEM-1型β-内酰胺酶耐药基因的方法。方法选择TEM-1型β-内酰胺酶耐药基因作为靶序列,设计合成引物和探针;收集呼吸道感染患者痰标本培养的鲍曼不动杆菌279株,并对其进行检测分析。结果采用荧光聚合酶链反应检测鳗曼不动杆菌TEM-1耐药基因灵敏度为102拷贝,279株鲍曼不动杆菌中检出47株携带TEM-1基因,检出率为16.8%。结论应用Taqman探针荧光聚合酶链反应能够快速、准确检测鲍曼不动杆菌TEM-1型β-内酰胺酶耐药基因。更多还原

【Abstract】 Objective To develop a real-time fluorescence PCR assay to detect the TEM-1 β-lactamase gene of Acinetobacter baumannii.Methods To design and synthesize the primers and TaqMan probe of the TEM-1 β-lactamase gene,279 isolates of Acinetobacter baumannii originating from sputum samples were analyzed.Results The sensitivity of the assay for he TEM-1 βlactamase gene was 10~2 copies.Among 279 samples,47 strains were positive for TEM-1 β-lactamase gene(16.8%,47/279).Conclusion The TaqMan PCR could rapidly and exactly detect the TEM-1 β-lactamase gene of Acinetobacter baumannii更多还原