【摘要】 目的探讨胆管癌中核因子kB(NFkB)表达及其与微血管形成的关系。方法应用免疫组化法检测1997～2003年50例胆管癌组织、27例癌旁组织及9例正常胆管组织的微血管密度(MVD)、血管内皮生长因子(VEGF)和NFkB的表达。结果NFkB在胆管癌和癌旁组织中阳性表达率分别为96.0%(48/50)和100.0%(27/27),高于正常组织44.4%(4/9)(P<0.01);NFkB强阳性表达组MVD高于弱阳性组和阴性组(F=12.662,P<0.01);NFkB表达与MVD、VEGF的表达均呈正相关(rs=0.542、0.660,P<0.01)。结论NFkB在胆管癌中高表达,与微血管形成呈正相关,NFkB可能通过调节VEGF等血管形成相关因子促进微血管形成。更多还原

【Abstract】 Objective To explore the expression of nuclear factor kappa B (NF-kB) and the relationship with angiogenesis in cholangiocarcinoma.Methods Microvessel density (MVD),expression of VEGF and NF-kB were investigated in 50 cases of cholangiocarcinoma,27 cases of para-tumor,and 9 cases of normal bile duct specimens by immunohistochemistry.Results Positive expressive rate of NF-kB in cholangiocarcinoma (48/50) and para-tumor (27/27) was significantly higher than that of normal bile duct tissue (4/9)(P<0.05).In NF-kB strong positive expression group,MVD was significantly higher than that in NF-kB weak positive group or in NF-kB negative group(F=12.662,P<0.01).Expression of NF-kB was positive relative with MVD (rs=0.542,P<0.01) and VEGF (rs=0.660,P<0.01).Conclusion NF-kB is up-regulated in cholangiocarcinoma and is positive relative with angiogenesis.NF-kB might promote angiogenesis through up-regulating VEGF expression in cholangiocarcinoma.更多还原