【摘要】 目的:测试三七总皂苷指纹图谱HPLC条件的耐用性。方法:在不同品牌的HPLC仪器和十八烷基硅烷键合硅胶色谱柱上,对梯度条件和柱温进行网格搜索。结果:在不同品牌的仪器、色谱柱上,可得到分离效果基本一致的指纹图谱,可以同时对人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1、人参皂苷Rd、人参皂苷Re 5个成分进行准确定量。结论:通过优化实验,可以保证三七总皂苷的HPLC指纹图谱在不同的仪器、不同品牌的色谱柱上重现,从而保证三七指纹图谱质量标准的顺利执行。更多还原

【Abstract】 AIM: To test the robustness of the HPLC conditions for the fingerprint of notoginsenosides.METHODS: Searching for gradient profile and column temperature by grid rake techniques on different band C-18 columns and HPLC instruments.RESULTS: The fingerprints from different band C-18 columns and HPLC instruments were similar and the HPLC conditions were suitable for determining notoginsenoside R₁,ginsenoside Rg₁,ginsenoside Re,ginsenoside Rb₁ and ginsenoside Rd simultaneously.CONCLUSION:By the optimization,fingerprints of notoginsenosides obtained by using different band C-18 columns and HPLC instruments are similar,thus the standard of notoginsenosides fingerprint can be performed reproductively.更多还原