【摘要】 将与牛血清白蛋白(BSA)偶联的阿维菌素人工合成抗原AVM-BSA免疫8周龄雌性BALB/c小鼠,利用杂交瘤技术获得了2株分泌抗阿维菌素特异性抗体的杂交瘤细胞株,分别命名为2F2和2H10。生物学特性鉴定的结果表明,2株杂交瘤细胞株诱生腹水的效价为1∶6 400,分泌的抗体亚型均为IgM;间接竞争酶联免疫吸附试验结果表明,抗体的50%抑制质量浓度(IC50)为101 ng/mL,2株单克隆抗体与伊维菌素、多拉菌素、红霉素和白霉素均无交叉反应,证实单克隆抗体2H10具有很强的特异性,可用于阿维菌素药物残留免疫分析方法的建立。更多还原

【Abstract】 Eight-week-old female BALB/c mice were immuned intraperitoneally with avermectin（AVM） coupled with bovine serum albumin（BSA）.Two hybridoma cell lines secreting monoclonal antibodies（McAbs） against AVM,which were named respectively as 2F₂ and 2H₁₀,were obtained by using the hybridoma technique.In results,subtype of the two McAbs was IgM,and titers of their ascetic fluids were 1∶6400 approximately in indirect ELISA.The two McAbs had no cross-reaction with ivermectin,doramectin,erythromycin and albomycin,respectively and their 50% of inhibition concentration was 101ng/mL in Ci-ELISA.The results showed that 2H₁₀ with high specificity will be useful for the development of methods for the immunologic determination of AVM residues.更多还原