【摘要】 利用RT-PCR和Western-blotting技术分析了胆固醇侧链裂解酶(P450scc)、3β-羟甾脱氢酶(3-βHSD)、C17-20裂解酶(P450c17)、StAR mRNA和蛋白在成年昆明小白鼠心脏、脾、肝、肾中的表达。结果显示,StAR mRNA及其蛋白、P450scc mRNA在以上组织中均有表达;3-βHSD在肾中有较强的表达,在其他组织的表达较弱;P450c17在上述组织中无明显的表达,但在睾丸中有明显的表达。这表明,心脏、脾、肝、肾均具有合成类固醇的能力,但合成类固醇的类型存在一定的差异。更多还原

【Abstract】 mRNA expression levels of cholesterol side chain decomposition enzyme(P450scc), 3β-hydroxysteroid dehydrogenase(3β-HSD),cytochrome P450 17 alpha-hydroxylase(P450c17)and steroidogenic acute regulatory protein(StAR) were examined by RT-PCR and the expressed protein levels of StAR were examined by Western-blotting analysis in nonsteroidogenesis tissues including the heart,spleen,liver and kidney of adult Kunming mice.In results,StAR mRNA and protein and P450scc mRNA were expressed in all tissues.3β-HSD had higher expression level in the kidney than those in the other 3 tissues.P450c17 was expressed in testis of mice.The results demonstrated that the 4 tissues had the ability of steroids biosynthesis,but the types of synthesized steroids were different among these tissues.更多还原