【摘要】 用甘肃棘豆草粉饲喂经苦马豆素(SW)-BSA免疫接种的山羊,检测分析了山羊血清中与中毒相关的酶的活性,以探讨SW-BSA免疫接种对动物机体组织器官的保护作用。将24只山羊随机分为免疫对照组、免疫攻毒组和未免疫攻毒对照组,免疫对照组和免疫攻毒组山羊接种SW-BSA,免疫攻毒组和未免疫攻毒对照组山羊按干重拌料饲喂10 g/(kg.d)甘肃棘豆草粉,检测血清谷草转氨酶(GOT)、谷丙转氨酶(GPT)、乳酸脱氢酶(LDH)、碱性磷酸酶(AKP)、尿素氮(BUN)和α-甘露糖苷酶(AMA)的变化。结果表明,免疫攻毒组山羊较未免疫攻毒对照组山羊LDH活性升高延缓28 d,AKP活性升高延缓14 d,AMA活性降低延缓21 d,BUN活性升高延缓14 d,GOT活性升高延缓28 d。这些酶活性延缓变化,说明SW-BSA免疫动物后,在甘肃棘豆攻毒后的30 d内,能够有效地延缓SW对山羊肝、心和肾等组织器官的损伤。更多还原

【Abstract】 24 goats were divided into immune control group,immune and poisoning group,poisoning control group randomly.Goats in the immune control group and immune poisoning group were vaccinated with SW-BSA.Goats in the immune and poisoning group,and the poisoning control group were fed with 10g/kg BW/d powder of O.kansuensis each day.The changes of serum GOT,GPT,LDH,AKP,BUN and AMA were examined.Results revealed that the increasing activity of LDH and GOT was delayed for 28 days,the increasing activity of AKP and BUN was delayed for 14 days,and the decreasing activity of AMA was delayed for 21 days in the immunized and poisoning goats compared with poisoning control goats.The results indicated that in 30 days,SW-BSA could prevent liver,kidney and heart of goats from injuring by SW effectively.更多还原