【摘要】 为研究微生态菌株Lactobacillusacidophilus对胃与小肠内胃酸、胃蛋白酶、胆汁盐以及胰蛋白酶的抗逆能力,以MRS为培养基,模拟人体正常胃环境pH1.60～5.00、胃蛋白酶分泌量0.592～1.482μg/mL,小肠胰蛋白酶活力14.24～72.32u/g及胆汁盐含量0.03%～0.30%时,LactobacillusacidophilusInd-1和Lactobacillusaci-dophilusLakcid在37℃温度下培养4h,用平板菌落计数法对两菌株进行活菌计数。结果表明:在pH1.60～5.00,胃蛋白酶0.592～1.482μg/mL条件下37℃培养4h,活菌数在108cfu/mL以上;在胰蛋白酶活力14.24～72.32u/g范围内、胆汁盐0.20%的条件下,活菌数可在107cfu/mL以上。说明Lactobacillusacidophilus能顺利通过胃与小肠而进入大肠,并存活下来。更多还原

【Abstract】 In order to investigate the survival kinetics of Lactobacillus acidophilus as microecological strains on gastric acid,pepsin,bile salt,trypsin in stomach and small intestine. Using MRS medium,simulating normal human gastrointestinal environment pH 1.60～5.00,pepsin 0.592～1.482 μg/mL,trypsin 14.24～72.32 u/g and bile salt 0.03%～0.30%,the living bacterial number(LBN)of L.acidophilus Ind-1 and L.acidophilus Lakcid which cultured at 37 ℃ for 4 h were counted by plate method. Results showed that LBN was above 108 cfu/mL at pH 1.60～5.00 and pepsin 0.592～1.482 μg/mL by culturing at 37 ℃ for 4 h;and above 107 cfu/mL at trypsin 14.24～72.32 u/g and bile salt 0.20%. It indicated that Lactobacillus acidophilus could go through the stomach and small intestine into large intestine and survive.更多还原