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产酸克雷伯氏菌胞外多糖单糖组成的气相色谱分析

Analysis of Monosaccharide Compositions in Exocellular Polysaccharide of Klebsiella Oxytoca XCH-1 By Gas Chromatography

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【作者】 刘红英薛长湖张辉刘喜梅张海莲

【Author】 Liu Hongying1,2 Xue Changhu2 Zhang Hui1 Liu Ximei1 Zhang Hailian1(1Ocean College,Agriculture University of Hebei,Qinhuangdao 0660032College of Food Science and Technology,Ocean University of China,Qingdao 266003)

【机构】 河北农业大学海洋学院中国海洋大学食品科学与工程学院河北农业大学海洋学院 秦皇岛066000青岛266003秦皇岛066000

【摘要】 从青岛海藻化工厂海带浸泡液中分离得到产酸克雷伯氏菌(KlebsiellaoxytocaXCH-1)。在有氧条件下,该菌在生长过程中产生大量的胞外多糖,并且粘度很大。目前国内外尚未有关于该菌胞外多糖理化性质的研究报道。实验用KlebsiellaoxytocaXCH-1胞外多糖为化学均一物质。用气相色谱分析法分析KlebsiellaoxytocaXCH-1胞外多糖单糖组分,结果表明其主要由D-葡萄糖、D-半乳糖和L-鼠李糖组成。

【Abstract】 The bacterium strain studied in this thesis was isolated and obtained from the water extract of Laminaria Japonica in Qingdao Algae Chemical Plant. The bacterium is examined as Klebsiella oxytoca XCH-1. It produces a large quantity of viscous exocellular polysaccharide during growing at oxygen supplied condition. At present,there is not any report at home and abroad about physical and chemical characteristics of exocellular polysaccharide of Klebsiella oxytoca XCH-1. In this paper,exocellular polysaccharide of Klebsiella oxytoca XCH-1,a single homogeneous material was analysed by Gas chromatography and the result indicates that the exocellular polysaccharide mainly consists of D-glucose,D-galactose and L-rhamnose.

【基金】 “863”青年基金项目资助课题(2001,AA625030)
  • 【文献出处】 中国食品学报 ,Journal of Chinese Institute of Food Science and Technology , 编辑部邮箱 ,2006年02期
  • 【分类号】TS201.2
  • 【被引频次】7
  • 【下载频次】298
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