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利用酶动力学拟合在密闭条件下香菇呼吸速率方程及米氏方程

The Estimation of Respiration Rate and Michaelis- Menten Models of Shiitake by Principle of Enzyme Kinetics in Closed System

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【作者】 谢晶刘晓丹

【Author】 Xie Jing Liu Xiaodan (College of Food Science and Technology, Shanghai Fisheries University, Shanghai 200090)

【机构】 上海水产大学食品学院上海水产大学食品学院 上海200090上海200090

【摘要】 对香菇分别在273K、283K和293K的密闭容器中氧气和二氧化碳随时间、浓度的变化进行了测定,根据酶动力学原理,利用非线性估计法、多重回归分析分别获得气体成分的变化率曲线和米式方程,从而获得相应的参数,求得反映呼吸状态的呼吸熵动态变化规律以及温度影响参数——活化能,并以此求出在任意温度、有氧呼吸气体环境条件下果蔬的最大呼吸速率,为气调包装系统设计提供理论依据。

【Abstract】 Experiments were conducted for the shiitake to measure the concentration of O2, CO2 varied by time in aclosed system. The respiration rate model and Michaelis- Menton model were developed separately, based on principle of enzyme kinetics. Meanwhile the parameters were obtained by analysis of nonlinear estimation regression and multiple linear regression so that respiration quotiety ( R.Q) and activity energy can be calculated, consequently, the maximum aerobic respiration rate could be gotten under any temperature, which is essential to the design of modified atmosphere package.

【基金】 上海市科技兴农重点攻关项目[沪农科攻字(2003)第6-3];上海市教委重点学科资助(No.T1102)
  • 【文献出处】 中国食品学报 ,Journal of Chinese Institute of Food Science and Technology , 编辑部邮箱 ,2006年01期
  • 【分类号】TS201
  • 【被引频次】25
  • 【下载频次】791
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