【摘要】 目的:研究缺血/再灌注损伤心肌肌浆网四种钙调控蛋白mRNA表达的变化。方法:将SD大鼠分为正常对照组和缺血/再灌注损伤组,采用Langendorff离体灌流技术,全心停灌15 min后行45 min复灌制备缺血/再灌注模型,记录心脏收缩功能各项参数(左室发展压、+dp/dt-max-、dp/dt-max),进行心律失常评分,同时对两组心肌标本进行半定量RT-PCR检测,测定SERCA、PLBI、P3R2和RyR2四种蛋白质的mRNA表达水平的变化。结果:与正常对照组比较,各时间点缺血再灌注心脏左室发展压、+dp/dt-max-、dp/dt-max均显著下降,再灌注阶段的心律失常评分明显增高,同时发现心脏SERCA,IP3R2,RyR2mRNA表达降低,然而PLB mRNA表达未出现变化。结论:缺血再灌注损伤造成心脏功能减退,心律失常增多,并可诱导心室肌钙调控蛋白SERCAI、P3R2、RyR2mRNA表达下调。更多还原

【Abstract】 Aim: To investigate the changes in the expression of four kinds of calcium regulatory proteins mRNA on the isolated ischemia/reperfusion（IR） hearts.Methods: The rat hearts were divided into two groups: control group and IR group which received 45 min exposure to Krebs-Henseleit solution after 15 min zero-flow global ischemia.The indexes of left ventricular function,such as LVDP,+dp/dt_max,-dp/dt_max,and an arrhythmia scoring system were compared between the two groups.The messenger ribonucleic acid（mRNA） amount of sarcoplasmic reticulum（SR） Ca²⁺ ATPase（SERCA）,phospholamban（PLB）,inositol 1,4,5-trisphosphate receptor₂（IP₃R₂） and ryanodine receptor₂（RyR₂） was measured by reverse transcription-polymerase chain reaction（RT-PCR） and norma（lized） to the mRNA levels of β-actin.Results: In the IR group,LVDP,+dp/dt_max and-dp/dt_min of the isolated hearts were depressed and the high rate of arrhythmias occurred during reperfusion.The levels of SERCA,IP₃R₂,RyR₂ mRNA were lower in the IR isola（ted） hearts group than those in the control group,while there was no difference in the level of phospholamban.Conclusion: These data suggest that myocardial ischemia/reperfusion can induce the depression of cardiac performance and an increased risk of arrhythmias,concomitanting with the decrease in SERCA、IP₃R₂、RyR₂ mRNA steady state levels.更多还原