【摘要】 为较准确地评估我国水稻主栽品种的一致性,挑选5个微卫星标记,对41个有2~7个来源的水稻品种进行了微卫星标记检测。结果表明,18个(43.90%)品种存在同一品种名称不同来源间的差异。进一步选择11个代表性水稻品种或杂交稻组合作较大样本分析(50个单株),它们在5个微卫星标记上的不一致率为0.0%~10.0%,平均为1.67%,每个微卫星标记的异质率变化幅度为0.36%~5.45%。11个品种中有6个(54.55%)在所有位点上完全一致,不一致率通常都不超过5%。因此,在构建水稻品种微卫星数据库时,必须慎重选择取样来源。当鉴别特定材料时,则应相应增加标记数。更多还原

【Abstract】 To have a better assessment of the within-variation variation of the major rice varieties in China,41 varieties from two to seven sources were assayed with a set of five well-chosen microsatellite markers,18 varieties(43.90%) of which showed heterogeneity among the different sources with the same name at 1 to 4 loci.Eleven varieties were further selected and analyzed with larger samples containing 50 individuals per variety.Non-uniformity of the varieties varies from 0.0-10.0% with an overall average of 1.67% on average five SSR loci,and the heterogeneity of the markers ranges from 0.36% to 5.45%.It also showed that 6 of the 11 typical rice varieties were completely uniform,and the non-uniformities within varieties across all loci were lower than 5%.Therefore,it is advisable that the sources of the materials should be selected carefully in constructing a microsatellite database,and that the number of markers should be correspondingly increased when identifying some specific materials.更多还原