【摘要】 目的探讨细胞免疫功能的变化与原因不明习惯性流产(unexplained habitual abortion,UHA)的发生及主动免疫治疗机制的关系。方法2002-08-2004-08汕头大学医学院第一附属医院采用流式细胞仪测定并比较112例UHA患者和30例正常已生育妇女及76例经主动免疫治疗后的UHA患者外周血CD3+、CD4+、CD8+、CD16+CD56+细胞亚群比例及CD4+/CD8+的比值;同时比较76例采用主动免疫治疗和36例未采用主动免疫治疗的UHA者再次妊娠成功率。结果UHA患者与正常已生育妇女比较,血中CD3+、CD16+CD56+细胞的百分率及CD4+/CD8+比值增高,差异有显著性意义(P<0·05)。主动免疫后CD3+、CD16+CD56+细胞百分率及CD4+/CD8+比值下降(P<0.05)。经主动免疫治疗的UHA者再次妊娠成功率为88.24%,对照组为31.25%,两组比较差异有非常显著性意义(P<0.01)。结论淋巴细胞亚群比例的改变与UHA的发生有关,主动免疫治疗可调节异常的细胞免疫功能,有利于提高再次妊娠成功率。更多还原

【Abstract】 Objective To investigate the association between the changes in cellular immunization function and unexplained habitual abortion（UHA） and the mechanism of active immunotherapy on UHA patients. Methods From August 2002 to August 2004,The flow cytometry （FCM） was used by First Affiliated Hospital,Shantou University Medical College to detect CD₃⁺、CD₄⁺、CD₈⁺T-lymphocyte and CD 16 ⁺CD 56 ⁺Natural Killer （NK） cell subsets and the ratio of CD₄⁺/CD₈⁺ of peripheral blood （PBL） .A total of 112 cases of UHA patients were studied and 30 cases of normal fertiled （NF） women as the control group; the percentages of T-lymphocyte and NK cell subsets before and after active immunotherapy were compared among 76 cases of UHA patients. The next successful pregnancy rates of UHA patients with（76 cases） and without（36 cases） active immunotherapy were compared. Results The percentages of CD₃⁺ and CD 16 ⁺CD 56 ⁺cell subsets as well as the ratio of CD₄⁺/CD₈⁺ of the UHA patients were significantly higher compared with those of NF cases （P<0.05）. After active immunotherapy, the percentages of CD₃⁺ and CD 56 + CD 16 + cell subsets as well as the ratio of CD₄⁺/CD₈⁺ of UHA cases were decreased significantly （P<0.05）. The next successful pregnancy rates of UHA cases with and without active immunotherapy were 88.24% and 31.25% respectively, the difference being significant, P<0.01. Conclusion The changes in the percentages of T-lympyocyte and NK cell subsets have something to do with UHA. Active immunotherapy can regulate the cell immunization function and increase the next successful pregnancy rate effectively.更多还原