【摘要】 目的构建四环素调控的SV40T转基因小鼠模型。方法同时显微注射外源基因p205-rtTA-C3和pTRE-Tag至FVB小鼠原核,注射受精卵移植到同期发情的假孕受体出生个体,经PCR和Southern检测获得阳性转基因小鼠。结果经PCR结合Southern检测得到rtTA和Tag双阳性转基因小鼠一只,rtTA单阳性两只和Tag单阳性一只。结论通过饮水给与四环素的双阳性小鼠可在卵巢中检测到Tag mRNA的表达。更多还原

【Abstract】 Objective To establish a tetracycline-dependent regulation of SV40 Tag transgenic mice.Methods\ p205-sv40Ta p205-rtTA-C3 and pTRE-Tag dual transgenic mice was generated by pro-nuclear microinjection and embryo transplantation.Positive founder mice were identified through both PCR and Southern blot.Results\ A rtTA and Tag dual positive transgenic mice,two rtTA single and one Tag single transgenic mice were confirmed by PCR and Southern Blot.The expression of Tag mRNA was detected by RT-PCR in the ovary of the dual transgenic mice which were administered with tetracycline in the drinking water.Conclusion\ The results of this study indicate that it is possible to establish a tetracycline-dependent regulation of SV40 Tag transgenic mice by administeration of tetracycline in the drinking water.更多还原