【摘要】 目的比较大鼠肠缺血再灌注损伤时,三七总皂苷(PNS)与谷氨酰胺(Gln)的肠屏障保护作用。方法将60只健康SD大鼠随机分为以下4组:(1)PNS组(n=20),给予PNS胃内灌服,每日3次,每次50mg/kg,共3天;(2)Gln组(n=20),给予Gln胃内灌服,每日3次,每次1g/kg,共3天;(3)单纯手术对照组(n=10),除不夹闭肠系膜上动脉(SMA),其余操作同模型对照组;(4)模型对照组(n=10)。均在最后一次给药2小时后进行手术,夹闭SMA造成肠缺血,1小时后恢复SMA血流,再灌注3小时后分别观察血浆内毒素水平,肝、脾和肠系膜淋巴结的细菌移位率及小肠组织病理学改变。结果(1)模型对照组、PNS组和Gln组大鼠肠道细菌移位率分别为73.33%、45.00%和46.67%,明显高于单纯手术对照组的3.33%(P均<0.01);而模型对照组的细菌移位率也明显高于PNS组和Gln组(P均<0.05);PNS组与Gln组之间差异无显著性(P>0.05)。(2)模型对照组、PNS组和Gln组大鼠血浆内毒素水平分别为(0.553±0.039)、(0.318±0.061)和(0.336±0.05)EU/ml,明显高于单纯手术对照组的(0.152±0.052)EU/ml(P均<0.01);而模型对照组的血浆内毒素水平也明显高于PNS组和Gln组(P均<0.05);PNS组与Gln组之间差异无显著性(P>0.05)。(3)电镜下可见模型对照组损伤较严重,PNS组与Gln组损伤较轻。光镜下chiu分级,PNS组与Gln组相比差异无显著性(P>0.05),但PNS组及Gln组与模型对照组相比损伤均明显减轻(P均<0.01)。结论PNS和Gln胃内灌服给药对缺血再灌注3小时的大鼠肠黏膜屏障具有一定保护作用,二者差异无显著性。更多还原

【Abstract】 Objective To compare the protective effect of saponins of panax notoginseng(PNS)with that of glutamine(Gln) on gut barrier in rats with intestinal ischemia-reperfusion (I/R) injury. Methods I/R injury rat model was established by occluding the superior mesenteric artery (SMA) for 1 hour and followed by reperfusion for 3 hours. Healthy SD rats were randomly divided into 4 groups: group A,(I/R rats,n=10),group B (sham-operation,n=10),group C (treated with PNS 50mg/kg for 3 days,q8h,n=20),and group D (treated with Gln 1g/kg for 3 days,q8h,n=20). Intestinal impairment was evaluated according to the mucosal histological changes under light and electron microscope and by measuring endotoxin concentration in plasma and ratios of translocation of bacteria in liver,spleen,and mesenteric lymph nodes(MLN). Results After 1 hour of ischemia and 3 hours of reperfusion,the endotoxin levels in plasma were significantly lower in group C and group D,which were (0.318±0.061)EU/ml and (0.336±0.057)EU/ml respectively,than that in group A,which was (0.553±0.039)EU/ml (all P<0.05). The ratios of bacterial translocation in liver,spleen,and MLN was significantly lower in group C (45.00%) and group D (46.67% ) than in group A (73.33%)(all P<0.05). Mucosal histological examinations under light and electron microscopes showed the fabric of intestinal tissue in group C and group D were fairly integrity and the mucosal of intestinal tissue were obviously injured in group A. No significant difference was found on all indexes between group C and group D. Conclusion Both PNS and Gln can protect intestinal mucosal barrier of rat with intestinal ischemia-reperfusion injury,and such protective effects were similar between PNS and Gln.更多还原