【摘要】 根据国外已发表的番鸭细小病毒(MDPV)FM株基因组核苷酸序列设计1对引物,应用PCR技术扩增MDPV FS株的VP2-VP3蛋白基因片段。将扩增后的VP2-VP3结构蛋白基因克隆到T载体上,MDPV FS株基因大小为1764bp,编码528个氨基酸,对插入片段进行序列测定。结果表明,我国分离的MDPV FS株基因序列与国外发表序列的同源性为98.6%,与已发表的YZ株同源性为99.5%,可见番鸭细小病毒结构蛋白基因较保守。更多还原

【Abstract】 A pair of primers were desiged to amplify the major structural protein(VP2-VP3)gene of muscovy duck parvovirus(MDPV) isolate FS,according to nucleotide suquences of MDPV isolate FM. The VP2-VP3 genes of FS was obtained by PCR and sequenced after being directly inserted into the T vector. The sequence analysis demonstrated that the VP2-VP3 genes of FS consisted of 1 764 bp,encoding 528 amino acids. Nucleotide homologies of VP2-VP3 genes of FM and YZ were 98.6% and 99.5%,Which indicated that the major structural protein genes were mere conservative.更多还原