【摘要】 目的探讨激光原位角膜磨镶术(laser in situ keratom ileusis,LASIK)术后角膜瓣下上皮细胞植入发生原因及Ⅳ型胶原与上皮植入后角膜混浊的关系。方法健康成年大耳白兔12只,随机取一眼为实验眼,作以鼻侧为基底160μm厚角膜瓣,机械刮取瓣周围角膜上皮细胞,并植于瓣下,分别于术后第1、3、7和30d处死实验动物取角膜,进行HE染色及免疫组织化学研究。结果术后裂隙灯下可见植入上皮处角膜瓣下混浊,HE染色切片光镜下可见层间上皮细胞生长,术后1周起围绕植入上皮细胞周围见Ⅳ型胶原异常强荧光染色。结论基底膜主要成分Ⅳ型胶原在基底膜外其他部位的异常沉积是LASIK术后上皮植入造成角膜混浊的主要原因。更多还原

【Abstract】 Objective To investigate the localization of collagen type Ⅳin cornea and its relationship to corneal opacity in epithelial ingrowth after LASIK. Methods Corneal flap was made on 12 rabbit eyes, and epithelial cells mechanically scraped from surroundings the flap were implanted underneath. The corneas were harvested 1,3,7 and 30 days following surgery, histological and immunohistochemical examination was performed. Results On slit-lamp examination, corneal opacity was observed in the area where epithelial cells were implanted. Histological study revealed clusters of epithelial cells at the flap and stromal bed interface. One week and one month post-operation intense immunoreactivity for collagen type Ⅳ was detected at the perimeters of the intrastromal epithelial island, but not in the ordinary interface area. Conclusions The marked deposition of collagen type Ⅳ surrounding the ingrowth epithelial cells indicated that it might be the essential component of interface haze in epithelial ingrowth after LASIK.更多还原