【摘要】 为探索有效的生物除磷方法,构建了聚磷激酶基因(ppk)的表达载体pET28a(+),并转化大肠杆菌(Escherichiacoli)BL21,获得了可过表达ppk基因的工程菌BL-PPK.RT-PCR结果表明,ppk基因在转化的大肠杆菌中得到了高效表达.聚磷试验结果表明,培养10h后,转化了ppk基因的大肠杆菌细胞内聚磷含量比对照菌株高20倍,而培养液中可溶性磷浓度为对照菌株的约1/9.更多还原

【Abstract】 For exploring an effective technique of phosphorus bioremoval,the express carrier pET28a(+)of polyphosphate kinase(ppk)gene was constructed and transformed as Escherichia coli BL21,and engineering bacteria BL-PPK capable to express ppk gene was obtained.The ppk gene in transformed Escherichia coli obtained highly effective expression.Experiment results of polyphosphate showed that the content of polyphosphate in the Escherichia coli cell transformed to ppk gene was 20 times higher than that in control bacteria after 10 h culture;while in culture liquor,the soluble phosphorus concentration was about 1/9 of the control bacteria.更多还原