【摘要】 正己烷/异丙醇为流动相的正相体系中,使用SupelcosilLC-CN氰基柱完全分离了氯氰等多种拟除虫菊酯农药的非对映异构体;使用SupelcosilLC-CN保护柱串联ChiralcelOD-H手性柱以及使用SupelcosilLC-CN氰基柱串联ChiralcelOD-H手性柱实现了氯氰等多种拟除虫菊酯类农药的对映异构体分离.流动相中异丙醇的比例对保留时间和分离度的影响较大,随着异丙醇比例的减少,保留时间和分离度逐渐增加;在0~30℃之间,柱温对手性识别能力无明显的影响;但对保留时间影响明显,随着柱温的增加,保留时间缩短.更多还原

【Abstract】 Separation of the diastereomers and enantiomers of synthetic pyrethroids was performed by high performance liquid gromatography (HPLC) with different columns in normal phase. The Supelcosil LC-CN column was applied to separate the diastereomers of commonly used pyrethroids such as cypermethrin etc.; the Supelcosil LC-CN precolumn and the Chiralcel OD-H column were chained to separate the enantiomers of pyrethroids such as cypermethrin etc.; the Supelcosil LC-CN column and the Chiralcel OD-H column were chained to separate the enantiomers of pyrethroids such as cypermethrin etc.. All diastereomers and enantiomers were sufficiently separated in a rather short time. The retention time and resolution of the diastereomers and enantiomers of pyrethroids were remarkably affected by the proportion of isopropyl alcohol in mobile phase. The resolution and retention time were increased while the proportion of isopropyl alcohol in mobile phase was decreased. There was no apparent change on the chiral recognization ability but observable change on the retention time when column temperature was increased from 0 to 30℃.更多还原