【摘要】 目的研究染料木素(Gen)对人前列腺癌细胞(PC-3)的影响。方法将PC-3细胞在DMEF-12培养液(含10%胎牛血清)中采用开放式单层贴壁培养,采用四甲基偶氮噻唑蓝(MTT)比色法、细胞核分裂指数、集落形成和3H-TdR掺入试验方法进行检测。染料木素剂量设为10,20,40,80μmol/L。以二甲基亚砜(DMSO)为溶剂对照。结果不同剂量的染料木素对PC-3细胞的生长增殖有不同的抑制作用。在MTT试验中,不同浓度染料木素对PC-3细胞的7d抑制率分别为27.7%,58.8%,72.3%,84.4%。在核分裂指数试验中,随着染料木素剂量的增高,其核分裂指数明显降低;遮集落形成试验中,随着染料木素剂量的增加,集落形成抑制作用增加;在3H-TdR掺入试验中,可见随着染料木素剂量的增高,3H-TdR掺入到PC-3细胞中明显的减少,与阴性对照组比较差异有统计学意义。结论染料木素对PC-3细胞的生长增殖有明显抑制作用,可能是染料木素抑制前列腺癌的机制之一。更多还原

【Abstract】 Objective To study the influence of Genistein on the growth of prostate carcinoma PC-3 cells in vitro.Methods Prostate cancer cells were grown in DMEF 12 medium supplemented with 10% bovine serum and 5?ml 10?000?U/ml of penicillin/streptomycin in an incubator maintained at 5% CO₂ and 37℃.Using cell culture tests（MTT test,index of cellular mitosis,Cloning formation and（）³H-TdR incorporation test）,We examined the growth of PC-3 cells at four concentrations genistein 10,20,40,80?μmol/L with a negative control（anhydrous alcohol and DMSO v/v）.Results Genistein could inhibied the growth of PC-3 cells.After treatment with various geinistein concentrations mnentioned above for 7 days,the inhibition rates were 27.7%,58.8%,72.3% and 84.4% respectively.With the increase of genistein concentrations,Mitotic index and Cloning formations were decreased in PC-3 cells and the quantity of（）³H-TdR incorporation was significantly decreased as compared with the negative control（P<0.05）.Conclusion Genistein can significantly inhibit the growth of PC-3 cells and it is probably one of the preventive mechanisms of genistein in prostate cancer.更多还原