【摘要】 目的探讨牡蛎提取物对四氧嘧啶所致小鼠胰岛细胞损伤的保护作用。方法50只小鼠随机分为模型组、3个实验组和对照组,每组10只;3个实验组给予不同剂量的牡蛎提取物灌胃[200,400,1 200 mg/(kg.bw)],对照组和模型组给予生理盐水,连续灌胃4周。然后模型组和3个实验组腹腔注射四氧嘧啶[200 mg/(kg.bw)],对照组注射生理盐水。72 h后,采集血液和胰腺,测定血糖、胰岛素、肿瘤坏死因子水平,并观察胰腺病理变化。结果牡蛎提取物组小鼠胰岛素水平明显高于模型组,血糖、肿瘤坏死因子水平则明显低于模型组;胰腺病理变化较模型组轻。结论牡蛎提取物对四氧嘧啶所致的小鼠胰腺细胞损伤有一定的保护作用。更多还原

【Abstract】 Objective To explore protective effect of oyster extract on pancreatic β cell damage induced by Alloxan in mice.Methods 50 mice were randomly divided into model group,three experimental groups and control with 10 each.Mice in three experimental groups were treated 20,400,1?200?mg/(kg·bw)oyster extract respectively by gavage for continuous 4 weeks,and those in control and model groups received an equivalent volume normal saline.Then the mice in three experimental and model groups were injected intraperitoneally with a single dose of alloxan[200?mg/(kg·bw)]in normal saline,and of equal volume of normal saline in control.72?h after injection,the blood and pancreas were collected.The levels of blood sugar,insulin and tumor necrosis factor were determined respectively,and the morphologic observation of pancreas also was made.Results Insulin levels of mice in the oyster extract groups were significantly higher than that of model group(P<0.05),while blood sugar and tumor necrosis factor in serum of mice in oyster extract groups were much lower than those in model group(P< 0.01),and pancreatic cell damage of mice in oyster extract groups were lighter than those of model group also.Conclusion Oyster extract had significant protective effect on the pancreatic β cell damage induced by Alloxan.更多还原