【摘要】 目的检测杀螟硫磷的环境雌激素活性,并探讨其作用机制。方法人乳腺癌细胞株(MCF-7)细胞在含10%小牛血清的RPMI1640培养基中进行半开放式贴壁培养,加受试物前2周,改在含10%去激素小牛血清的无酚红RPMI1640培养基中培养。试验设溶剂对照组、阳性对照组及杀螟硫磷5个不同浓度实验组,采用MCF-7细胞增殖试验、四甲基偶氮噻唑蓝(MTT)试验和生长曲线观察杀螟硫磷对MCF-7细胞增殖的影响,并通过雌激素受体(ER)结合试验分析杀螟硫磷与ER的结合情况。结果杀螟硫磷在10-9~10-6mol/L剂量范围内可诱导MCF-7细胞增殖,差异有统计学意义(P<0.05),且存在剂量-效应关系。杀螟硫磷能与ER结合,抑制125I-E2与ER结合。结论杀螟硫磷具有环境雌激素活性,其拟雌激素作用由ER介导而产生。更多还原

【Abstract】 Objective To evaluate the environmental estrogenic activity of the pesticide fenitrothion and study the possible mechanism.Methods Human breast cancer cell strain MCF-7 cells were maintained in RPMI1640 medium containing 10% bovine serum.Two weeks before the addition of Fenitrothion,the cells were transformed into phenol red-free RPMI1640 medium containing 10% charcoal/dextran treated bovine serum.There were seven groups,vehicle control group,positive group and five Fenitrothion groups.MCF-7 cells proliferation test,MTT test and growth curve were used to assess the effect on the proliferation of MCF-7 cells by fenitrothion and estrogen receptor（ER） binding test was used to analyze the binding ability of fenitrothion with ER.Results Fenitrothion could induce the proliferation of MCF-7 cells at 10^-7?mol/L～10^-5?mol/L with dose-effect relationship,and this effect was significantly different from the vehicle control（P<0.05 or P<0.01）.Fenitrothion could bind with ER and inhibit the bind of（）¹²⁵I-E₂ with ER.Conclusion Fenitrothion has environmental estrogenic activity which was mediated by ER.更多还原