【摘要】 目的探索依布硒啉(Ebs)和谷胱甘肽(GSH)对酒精依赖大鼠过氧化损伤的抑制作用和效果。方法测定第3周末酒精依赖大鼠血清中的谷胱甘肽过氧化物酶(GPx)活性和脂质过氧化物丙二醛(MDA)生成量,以GPx与MDA比值反映过氧化抑制作用。将服药组的GPx与MDA比值减去阳性组GPx与MDA比值除以服药组GPx与MDA比值的百分率,得出抑制效率。结果Ebs 0.1~10 mg/kg与GSH5~10 mg/kg联合作用的过氧化抑制效率在40.0%~65.7%之间(P均<0.01),显示剂量与功效关系(r=0.693,P<0.01)。Ebs 5~10 mg/kg的抑制效率为36.7%~45.3%。结论酒精过氧化损伤时的Ebs抗氧化作用除依赖辅助因子GSH外,还可能通过其他途径产生。更多还原

【Abstract】 Objective To investigate inhibitory effect of Ebselen and glutathione（GSH）on peroxidation of Alcohol-dependent rats.Methods Serum glutathione peroxidase（GPx） activity and lipid peroxide（LPO）content and malondialdehyde（MDA） of alcohol-dependent rats were detected in end of 3 weeks after exposure,and ratio of GPx activity to MDA was applied to index the inhibitory effect of peroxidation.The ratio of each ebselen-exposure group with different concentration substracted the ratio of positive control group,the result was divided by the ratio of ebselen-exposure group and a percentage was gotten which represented the efficiency of inhibition.Results Ebselen and GSH were administerd simultaneously and a synergistic effect could be invesgated when the concentration of Ebselen and GSH varied from 0.5mg/kg to 10mg/kg and 5～10mg/kg,respectively.The efficiency of inhibition increased from 40.0% to 65.7%(P_ar<0.01),which displayed a dose-response relationship（r=0.693,P<0.01）.When ebselen of different concentration（5～10?mg/kg）was administered singly,the inhibitory effect could also be obtainde which varied from 36.7% to 45.3%.Conclusion Ebselen can display antioxidative effect with the co-factor GSH,and other pathways may also exert in the mechanism of antioxidant effect of ebselen.更多还原