【摘要】 目的研究断乳后饲料构成对大鼠肝脏肉碱棕榈酰转移酶-I(CPT-I)mRNA表达的影响。方法新生Wistar雄性大鼠24 d断乳,按体重随机分为A、B、C、D 4组,分别喂饲高碳水化合物、高蛋白质、高不饱和脂肪酸和高饱和脂肪酸构成饲料3周后,基础饲料喂养2周,A组按体重随机再分为A1、A2 2组。A1组喂饲基础饲料作为对照组,A2和B、C、D 4组给予高脂饲料喂养6周,A2组作为高碳水化合物组。动态检测大鼠的体重、体脂含量、血糖及肝脏肉碱棕榈酰转移酶-I mRNA水平。结果实验末期,C组大鼠体重、体脂含量和血糖均明显低于A2组(P<0.05);B组大鼠体重和体脂含量明显低于A2组(P<0.05),但血糖水平高于A2组;D组大鼠体重明显低于A2组(P<0.05),但血糖和体脂含量与A2组差异无统计学意义(P>0.05)。动态观察表明,C组大鼠CPT-I mRNA水平持续升高,且在实验末期明显高于其他组。结论断乳后进食高不饱和脂肪酸构成饲料可能通过持续增加CPT-I基因表达,抑制大鼠肥胖形成。更多还原

【Abstract】 Objective To study the effects of postweaning dietary compositions on hepatic CPT-I gene expression in rats.Methods Male Wistar rats weaned on 24?d were randomly divided into A,B,C,D groups and were fed a high carbohydrate,high protein,high unsaturated fatty acid and high saturated fatty acid diet respectively.After three weeks,all rats were assigned to basic diet for 2 weeks,then rats from A group were randomly divided into groups A 1 and A2.A 1 was fed on basic diet continuously.Groups A2,B,C,and D were assigned to high-fat diet for six weeks.Eightrats from each group were sacrificed at the end of experiment to detect body weight,body fat content and blood glucose level.RT-PCR were used to determine the expression of CPT-I in liver tissue.Results Body weight,body fat and blood glucose level in C group were significantly lower than those in A2 on llw(P<0.05).The body weight,body fat content in B group were significantly lower than that in A2(P<0.05).However,blood glucose level was higher than A2 on1 lw(P>0.05).The body weight in D group was significantly lower than that in A2(P<0.05),but body fat content and blood glucose levels were not significantly lower than A2 on 11w(P>0.05).The CPT-I mRNA level in C group was persistently increased to be the highest among all groupsduring experiment and higher than that of other groups at the end of the experiment significantly.Conclusion Feeding on high unsaturated-fatty acid diet after weaning may significantly inhibit the obesity occurance,improve the abnormal blood glucose level in rats fed with high-fat diet.Persistently increased CPT-I gene expression may be involved in inhibiting later obesity by early nutrition.更多还原