【摘要】 目的探讨不同标本中产志贺样毒素大肠埃希菌(STEC)的分布特征。方法采集动物粪便、肉类食品和排污口污泥样品,常规分离大肠埃希菌,血清学分型,PCR鉴定产志贺样毒素(stxl,stx2)菌株。结果293份标本中鉴定出8株STEC,1株为产志贺毒素O157:H7型,2株为不产志贺毒素O157:H7型,5株为产志贺毒素非O157:H7型。结论STEC存在于不同来源的标本中,菌株表型与毒力因子存在一定差异。更多还原

【Abstract】 Objective To explore the distribution and characterization of Shiga toxin-producing E.coli from animal feces,meat food and contaminate mud.Methods Routine method was used to isolate E.coli,strains of Shiga toxin1-2 producing were detected by PCR assay;and types were analyzed by serological technique.Results Of all samples,8 isolates were identified as Shiga toxin-producing E.coli,one strain was identified as EHEC O157:H7,two strains as EHEC O157:H7 with no Shiga toxin and five strains as no-EHEC O157:H7 with producing Shiga toxin.Conclusion STEC could be separated from different samples,and there was slightly distance between types and pathogenic factors.更多还原