【摘要】 目的评价北京市儿童进行乙肝疫苗加强免疫接种后抗-HBs的应答反应水平。方法采取多阶段整群系统抽样选择已进行乙肝疫苗初免的3~12岁儿童,采用固相放射免疫(SPRIA)方法,检测血清HBsAg、抗-HBs和抗-HBc水平,其中抗-HBs<10 Iu/L者进行不同剂量的己肝疫苗加强免疫,观察加强免疫后的抗-HBs。结果2 491名3~12岁儿童的抗-HBs阴性率为48.25%(CI%=35.15~64.26);对其中726名儿童进行己肝疫苗加强免疫后,接种2.5和5μg疫苗的儿童抗-HBs的阳转率分别为90.6%和90.9%,GMT分别为85.69和87.59 Iu/L,(χ2=0.02,P=0.89;t=0.36,P=0.72)。加强免疫后4~5周,平均抗-HBs的阳转率为92.8%,GMT为84.55 Iu/L;免后6~22周采血,平均抗-HBs的阳转率为86.66%,GMT为91.39 Iu/L,2个时间段的抗-HBs的阳转率差异有统计学意义(χ2=7.47,P=0.006)。结论3~12岁完成乙肝疫苗基础免疫的儿童,在抗体消失后给予一定剂量的乙肝疫苗进行加强免疫,其抗-HBs反应良好,表明机体存在对乙肝病毒有回忆反应,可能是维持乙肝疫苗长期保护效果的原因之一。更多还原

【Abstract】 Objective To evaluate the character of the reminiscent responsion of the Hepatitis B vaccmebooster immunization on the children who were anti-HBs negative in Beijing.Methods The multiple stratified cluster sampling was used in this epidemiological survey.The sampling children’s blood serum HBsAg,anti-HBs and anti-HBc were checked and measured by the Solid Phase Radioimmunoassay(SPRIA).The children whose anti-HBs serological titer(GMT)was lower than 10?Iu/L had the Hepatitis B vaccine booster immunization in two different dosages.Results 2?419 3～12 years-old children who were immunized by the Hepatitis B vaccine in the infant period were surveyed and the total anti-HBs negative rate was 48.25%(CI%,35.15～64.26).725 children had the Hepatitis B vaccine booster immunization and the anti-HBs positive rate was observed.The anti-HBs positive rate of the children who were immunized in 2.5?μg and 5.0?μg Hepatitis B vaccine was 90.6% and 90.9% respectively.The GMT was 85.69 and 87.59?Iu/L respectively.The anti-HBs positive rate was also analyzed according to the different time of the blood sampling.And there were both no statistical significances between the two dosages(χ~2=0.02,P=0.89;t=0.36,P=0.72).The average anti-HBs positive rate was 92.80% and the GMT was 84.55?Iu/L after 4～5 weeks.The average anti-HBs positive rate was 86.66%and the GMT was 91.39?Iu/L after 6～22 weeks.There were statistical significance for the average anti-HBs positive rate(χ~2=7.47,P=0.006) and no statistical significance for the anti-HBs GMT between the different blood samplmg time(t=1.09,p=0.28).Conclusion For the 3～12 years-old children who had finished the basic immunization of Hepatitis B vaccine in the infant period,a little dose of Hepatitis B vaccine could make responsion strongly in their bodies after the antibody died away.It showed that there was remimscent responsion for the Hepatitis B virus in their bodies.This reminiscent responsion could be the main cause that made people have the long-term protection of Hepatitis B vaccine.更多还原