【摘要】 目的探讨肉类食品中变形杆菌第1类整合子的分布、特征及所含基因盒的种类。方法常规方法分离变形杆菌;纸片扩散法对9种抗生素进行耐药性监测和分析;PCR鉴定1类整合子;阳性株PCR产物测序并对结果进行分析。结果91份标本中共鉴定出27株变形杆菌,有13株(48.1%)对至少4种以上抗生素表现多重耐药,耐药谱为复合磺胺-氨苄西林-红霉素-四环素-链霉素。13株中有3株鉴定出1类整合子,2株携带0.75和1.8 kb 2个整合子。1株携带1.0 kb整合子,PCR产物测序,1.0 kb整合子携带aadA2基因盒,传递对氨基糖苷类抗生素的耐药性。结论肉类食品中变形杆菌携带第1类整合子,1.8 kb整合子携带pse-1-aadA1-dfrA14基金盒,传递对β-内酰胺类、磺胺类、氨基糖苷类抗生素的耐药性。携带复杂的基因盒,决定着对β-内酰胺类、氨基糖苷类和磺胺类抗生素的耐药性。更多还原

【Abstract】 Objective To explore the distribution and character of class 1 integrons in Porteues from meats,and to elucidate the status of genecassettes.Methods Routine method was used to isolate Proteues and antibiotics susceptibility was tested by the disk diffusion method;class 1 integron was detected by PCR assay;PCR products were sequenced and analyzed.Results Of 91 samples,27 isolates were identified,and 13 isolates were multiple-drug resistant.The antibiogram was sulfamethoxazole-trimethoprim,ampicillin,streptomycin,tetracycline,erythromycin.3 of 14 isolates carried class 1 integrons;and two strains laboured two integrons with 1.8?kb and 0.75?kb,one strain laboured 1.0?kb integron.The sequenced PCR conduct demonstrated that the 1.8?kb integron laboured pse-1-aadA1-dfrA14 gene cassettes confered the resistance of β-lactamas,sulfamethoxazole-trimethoprim,and aminoglycoside;the 1.0?kb integron laboured aadA1 gene cassette confered the resistance of aminoglycoside.Conclusion The different kinds of class 1 integrons exists in proteues from meats,and determines the multiple-resistant antibiotics.更多还原