【摘要】 为提高新城疫临床诊断的准确率,建立了新城疫病毒浓缩方法,将粪便及脑、肺等器官组织病料中的新城疫病毒经特异性抗体致敏的金黄色葡萄球菌(SPA菌)吸附浓缩后,用RT-PCR进行检测。结果表明,所建立的新城疫病毒浓缩方法应用于临床诊断后,可显著提高检测敏感性,从而提高临床诊断的准确率。更多还原

【Abstract】 In order to increase diagnostic accuracy, NDV was concentrated by absorbtion of NDV in feces, homogenate of clinical brain and lung tissue to the sensitized staphylococcal protein A with specific antibody. Then the staphylococcus aureus were centrifuged and applied for RT-PCR. The results showed that the NDV concentration method can greatly improve sensitivity of the test, consequently, increase clinical diagnostic accuracy.更多还原