【摘要】 目的:检测hTERT基因反义核酸对A2780细胞端粒酶活性的影响及其机制。方法:TRAP法检测端粒酶活性,流式细胞仪检测hTERT蛋白表达。结果:检测端粒酶活性,A2780细胞吸光度A值为(0.492±0.051),hTERT反义核酸作用48 h A值降为(0.351±0.051),hTERT反义核酸作用72 h A值降为(0.238±0.024)检测hTERT蛋白表达,A2780细胞hTERT蛋白阳性率(89.513±3.389)%,hTERT反义核酸作用48hhTERT蛋白阳性率低至77.237±2.872%,hTERT反义核酸作用72 h hTERT蛋白阳性率低至(47.767±1.226)%。而hTERT正义核酸无上述作用。hTERT反义核酸作用A2780细胞48 h、72h对hTERTmRNA表达无影响。结论:hTERT反义核酸降低A2780细胞端粒酶活性,机制可能是降低hTERT蛋白表达量。更多还原

【Abstract】 Objective:To observe the effect of antisense oligodeoxynucleotide of human telomerase reverse transcriptase(hTERT) on the telomerase activity in A2780 cells and its mechanism.Methods:The telomerase activity was detected by TRAP.hTERT protein expressions were detected by flowcytometry.Results:The Absorbency of the cells(A values) was used to represent the telomerase activity.At 48 and 72 h,the A values of the A2780 cells treated with antisense oligodeoxynucleotide of hTERT decreased to 0.351±0.051 and 0.238±0.024,respectively,compared to the A value of 0.492±0.051 in the control cells without treatment(P<0.05).The positive rates of hTERT protein expression in A2780 cells treated with antisense oligodeoxynucleotide of hTERT at 48 and 72 h were 77.237%±2.872% and 47.767 %±1.226%,respectively,compared to the positive rate of 89.513%±3.389% in the control cells without treatment(P<(0.05).)No change of hTERT protein expression was observed when sense oligodeoxynucleotide of hTERT was used.Conclusion:Antisense oligodeoxynucleotide of hTERT could downregulate the telomerase activity and hTERT protein expression in A2780 cells.更多还原